The Correlation of Treg Cells (CD4+CD25+) with Viral Load between HIV Patients and TB-HIV Patients in Semarang Indonesia

Muchlis Achsan Udji, Yanri W Subronto, Marsetyawan HNE Soesatyo, Soeharyo Hadisaputro, Umi Intansari, Iva Puspitasari, Dharminto Dharminto, Suhartono Damas


The number of HIV-AIDS patients in Indonesia has considerably increased. The life expectancy of HIV-AIDS patients gets longer in the presence of antiretroviral treatment (ARV). Most opportunistic infections of HIV infection are found to be Tuberculosis (TB-HIV). Antiretroviral and anti-tuberculosis treatment will improve the quality of life of people living with HIV-AIDS (PLWHA). Regulatory T cells (Tregs) were suggested to play a role in immune response of HIV and TB-HIV. This study aims to investigate the response of Tregs of patients with HIV and TB-HIV, their clinical changes, and other immunological aspects such as CD4+ T cells. The viral load in peripheral blood among two groups after receiving antiretroviral treatment (ARV) was also measured.

The cohort study was carried out in Dr Kariadi Hospital Semarang from January 2012 to June 2013 on 52 HIV patients and 40 TB-HIV patients who were observed for one year of antiretroviral and antituberculosis treatment. Five HIV patients and 5 TB-HIV patients died during the studies.  The number of Tregs (CD4+CD25+) was assessed by flowcytometer (FACS), viral load was done by measuring HIV-RNA in peripheral blood of patients with HIV and TB-HIV after one year antiretroviral treatment.

The results indicate that the number of Tregs was significantly different between the two groups at the beginning of treatment (p = 0.001). The number of Treg was increased in the HIV patients and was decreased in the TB-HIV patients after antiretroviral treatment. However, there was inverse relationship between the number of Tregs (CD4+ CD25+) and CD4+ T cells. There was positive relationship between the number of Tregs with viral load of HIV patients and TB-HIV patients after antiretroviral Treatment.

In conclusions, the number of Tregs (CD4 + CD25 +) may be suggested as a factor to determine progression of the diseases beside CD4+ T cells and viral load.


Treg cells (CD4 + CD25 +); CD4 T cell; viral load; HIV; TB-HIV

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Indonesian Ministry of Health, AIDS National Data June 2013. Jakarta.

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