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Fabrication of Photolithography Micro-grating pattern for DNA Optical Biosensors

Naphat Chathirat

Abstract


An optical biosensor device is a grating pattern structure fabricated using a Microelectromechanical system (MEMS), which is a photolithography process technique. This research describes the detection of biomolecular mass on the surface of this sensor device. Biomolecular mass consists of an amine functional group, EDC/NHS, Streptavidin in PBS 7.4, and biotin-ssDNA. When
molecules are attached to the surface of the devicce, the reflected wavelength is shifted due to the change of the optical path of light that is coupled into the grating structure. Therefore, the extent of the peak wavelength shift can be used to determine the amount of bound reagents and analyte. The advantages of this device are that it works with less bio-molecular mass than other methods and can be used at room temperature.

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References


L. Sergey Edward, MEMS AND NEMS Systems, Devices, and Structures, CRC Press, Boca Raton, LLC, 2002.

C. Brian, P. Li, L. Bo, and P. Jane, “Colorimetric resonant reflection as a direct biochemical assay technique”, Sensors and Actuators B, vol. 81,pp. 316-328, 2002.

R. Jenison, S. Yang, A. Haeberli, and B. Polisky, “Interference-based detection of nucleic acid targets on optically coated silicon”, Nature Biotechno, vol. 19, pp. 62-65, 2001.

V.S.-Y. Lin, K.Motesharei, K.-P.S. Dancil, M.J. Sailor,M.R. Ghadiri, “A porous silicon-based optical interferometric biosensor”, Science, vol. 278, pp. 840-843, 1997.

Evan Thrush, Ofer Levi, “Integrated bio-fluorescence sensor”, Journal of Chromatography A, vol. 1013, pp. 103-110, 2003.

A. Cunningham, Analytical Biosensors, Wiley, New York, 1998.

Bo Lin, Jean Qiu, and C. Brian, “A label-free optical technique for detecting small molecule interactions”, Biosensors and Bioelectronics, vol.17, pp. 827-834, 2002.

Leo L. Chan, Brian T. Cunningham, Member IEEE, Peter Y. Li, and Derek Puff, “A Self-Referencing Method for Microplate Label-Free Photonic-Crystal Biosensors”, IEEE SENSORS JOURNAL, vol.6, no.6,December 2006.

C.E. Jordan, R.M. Corn, “Surface plasmon resonance imaging measurements of electrostatic biopolymer adsorption onto chemically modified gold surfaces,” Anal. Chem, vol. 69, pp. 1449-1456, 1997.

F. Morhard, J. Pipper, R. Dahint, and M. Grunze, “Immobilization of antibodies in micropatterns for cell detection by optical diffraction”, Sensors and Actuators B, vol. 70, pp. 232-242, 2000.

G. Jin, P. Tengvall, I. Lundstrom, and H. Arwin, “A biosensor concept based on imaging ellipsometry for visualization of biomolecular interactions”, Anal. Biochem., vol. 232, pp. 69-72, 1995.

A. Brecht, G. Gauglitz, “Optical probes and transducers”, Biosens. Bioelectron., vol. 10, pp. 923-936, 1995.

Germain Chartier, Introduction to Optics, Springer, 2005.

R. Magnusson and S.S. Wang, “New principle for optical filters”, Appl. Phys. Lett., vol. 61, pp. 1022-1024, 1992.

C. Thirstrup, W. Zong, “Diffractive optical coupling element for surface Plasmon resonance sensors”, Sensors and Actuators B , vol. 100, pp. 298-308, 2004.

Hiroki Yakota, Forrester Johnson, “ A new method for straightening DNA molecules for optical restriction mapping”, Nucleic Acids Research, vol. 25, pp. 1064-1070, 1997.




DOI: http://dx.doi.org/10.21535%2FProICIUS.2010.v6.446

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